Hairy root culture of chinese medicinal plant tri chosanthes kirilowii have been seen producing trichosanthin a ribosome inactivating proteins can inhibit multiplication of the human immuno deficiency hiv the aids virus in recent year hairy root culture has emerged as useful to study the biology and engineering of plant chemical production. The production of hairy root culture by soil bacterium agrobacterium rhizogene mediated gene transformation using plant tissue culture hairy roots induced when explants of plant infected with root inducing plasmid of agrobacterium rhizogenes t dna transmit in plant cell and express along with plant genome hairy root culture. Bensaddek et al 2008 2 induction and growth of hairy roots for the production of medicinal compounds lamine bensaddek1 maria luisa villarreal2 and marc andre fliniaux1 1laboratoire de phytotechnologie ea 3900 universite de picardie jules verne 80000 amiens france 2centro de investigacion en biotecnologia universidad autonoma del estado de morelos uaem cp 62210. It serves as a model system for plant metabolism and physiology and utilized as a technical alternative to plant cell suspension culture moreover hairy root culture plays a significant role in design the principle for plant metabolic engineering germplasm conservation expression of foreign protein and phytoremedaition. Hairy root culture hrc based in vitro production system has become a promising biotechnological approach in recent years the hairy root formation is the result of agrobacterium rhizogenes induced pathogenesis in plants characterized by high growth rate growth in hormone free media besides genetic stability these roots can imitate intact
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